| 丙型肝炎病毒C33_c单克隆抗体的研制和鉴定 |
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| 引用本文： | 秘晓林,魏庆兰.丙型肝炎病毒C33_c单克隆抗体的研制和鉴定[J].微生物学免疫学进展,1996,24(4):17-21. |
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| 作者姓名： | 秘晓林 魏庆兰 |
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| 作者单位： | 卫生部兰州生物制品研究所 |
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| 摘 要： | 用丙型肝炎病毒重组蛋白Ｃ３３_ｃ抗原免疫ＢＡＬＢ／ｃ小鼠，运用杂交瘤技术成功地建立了７株能稳定分泌抗Ｃ３３_ｃ单克隆抗体的杂交瘤细胞１Ｈ６Ｄ２、２Ｇ１Ａ６、３Ａ４Ａ８、３Ｅ３Ｅ７、４Ｇ１２Ｃ１０、４Ａ１０Ｃ２、５Ｆ４Ｂ６．试验结果表明，７株ＭｃＡｂｓ具有良好的ＨＣＶ特异性，间接ＥＬＩＳＡ法测得小鼠腹水ＭｃＡｂ效价为１：１０￣４－１：４×１０￣４；竞争抑制实验和相加指数测定证实７株ＭｃＡｂｓ识别相关的抗原表位；７株ＭｃＡｂｓ中１株为ＩｇＭ（５Ｆ４Ｂ６），其它６株为ＩｇＧ（２ａ）。
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| 关 键 词： | 丙型肝炎病毒，单克隆抗体，C33_c抗原 |
Preparation of Monoclonal Antibodies to C33c Antigen of Hepatitis C Virus |
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| Bi Xiaolin,Wei Qinglan,Liu Xinming.Preparation of Monoclonal Antibodies to C33c Antigen of Hepatitis C Virus[J].Progress In Microbiology and Immunology,1996,24(4):17-21. |
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| Authors: | Bi Xiaolin Wei Qinglan Liu Xinming |
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| Abstract: | Seven mouse hybridoma cell lines secreting monoclonal antibodies to HCV C33 c antigen were prepared.Of the seven McAbs,six were IgG 2a ,one was IgM.The results showed that seven McAbs had a good specificity.Competitive inhibition test and additive index test proved that seven McAbs recognize the same binding site |
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| Keywords: | HCV McAb C33 c antigen |
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