Lateral enzyme topology in the rough endoplasmic reticulum of rat liver |
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| Authors: | Eriksson Lennart C Bergstrand Anders Dallner Gustav |
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| Institution: | (1) Department of Pathology at Huddinge Hospital, Karolinska Institutet and Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, Stockholm, Sweden;(2) Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, Fack, S-10405 Stockholm, Sweden |
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| Abstract: | Summary Rough microsomes were subfractionated on the basis of different properties in order to investigate the nature and extent of the enzyme heterogeneity of these vesicles. A discontinuous gradient, containing monovalent cations allowed the separation of a ribosome-poor membrane fraction which was enriched in electron transport enzymes and relatively poor in phosphatases. Zonal centrifugation on a stabilizing gradient separated 3 fractions characterized by enrichment of electron transport enzymes, glucose-6-phosphatase and adenosinetriphosphatase, respectively. An essentially similar pattern was seen when ribosomes were removed with EDTA and the denuded vesicles subfractionated on a sucrose gradient. Rough microsomes from phenobarbitaltreated rats exhibited the same pattern both qualitatively and quantitatively. It appears that electron transport enzymes and two types of phosphatases are heterogeneously distributed among rough microsomal vesicles.This work has been supported by grants from the Swedish Medical Research Council. The authors wish to thank Mrs. Ulla-Britta Torndal for her valuable technical assistance |
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| Keywords: | Microsomes Subfractionation Zonal centrifugation Heterogeneity Biogenesis Phenobarbital Surface charge Enzyme pattern |
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