| 马铃薯单双三价抗病毒基因表达载体的构建 |
| |
| 引用本文： | 崔晓江,彭学贤,沈禹飞,莽克强.马铃薯单双三价抗病毒基因表达载体的构建[J].生物工程学报,1994,10(2):185-189. |
| |
| 作者姓名： | 崔晓江 彭学贤 沈禹飞 莽克强 |
| |
| 作者单位： | 中国科学院微生物研究所，北京100080 |
| |
| 基金项目： | 本工作属中国科学院“八五”重大攻关项目 |
| |
| 摘 要： | 马铃薯Y病毒(PVY)、X病毒(PVX)和卷叶病毒(PLRV)引起的病害是造成我国马铃薯退化的主要原因,严重危害我国的马铃薯生产。PVY和PVX或PVY和PLRV混合侵染带来的损失远远大于各病毒单独侵染。国外科学家通过在马铃薯植株体内表达病毒外壳蛋白(CP)基因来减缓病毒病害的发生已取得相当的成功。 我们从河北省坝上地区农科所试验田中采集PLRV感病材料Burbank及87-1,参照文献提取病毒RNA并以其为模板,反转录合成cDNA。根据PLRV澳大利亚分离物已发表的序列,设计并
|
| 关 键 词： | 马铃薯 抗病毒基因 载体 基因表达 |
Cloning and Sequencing of PLRV Coat Protein Gene and Construction of Vectors for.Transgenic Potato Resistant to Coinfection of PVY, PVX and PLRV |
| |
| Cui Xiaojiang Peng Xuexian Shen Yufei Mang Keqiang.Cloning and Sequencing of PLRV Coat Protein Gene and Construction of Vectors for.Transgenic Potato Resistant to Coinfection of PVY, PVX and PLRV[J].Chinese Journal of Biotechnology,1994,10(2):185-189. |
| |
| Authors: | Cui Xiaojiang Peng Xuexian Shen Yufei Mang Keqiang |
| |
| Abstract: | The coat protein gene of potato leaf roll virus (Zhangjakou isolate) was cloned by PCR technology and sequenced. Comparisons of the nucleotide and deduced amino acid sequences of the PLRV coat protein gene with those of some reported isolates reveal over 97% homology between them.
In order to acquire transgenic potato resistant to coinfection of PVY, PVX and PLRV, we managed to put the three virus coat protein genes driven by the enhanced 35S promoter respectively in one plant expression vector. |
| |
| Keywords: | Potato leafroll virus coat protein coinfection transgenic potato |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《生物工程学报》浏览原始摘要信息 |
| 点击此处可从《生物工程学报》下载免费的PDF全文 |
