Endophytic Bacterial Communities in Ginseng and their Antifungal Activity Against Pathogens |
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Authors: | Kye Man Cho Su Young Hong Sun Mi Lee Yong Hee Kim Goon Gjung Kahng Yong Pyo Lim Hoon Kim Han Dae Yun |
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Institution: | (1) Division of Applied Life Science, Gyeongsang National University, Chinju, 660-701, Republic of Korea;(2) Research Institute of Agriculture and Life Science, Gyeongsang National University, Chinju, 660-701, Republic of Korea;(3) Department of Horticulture, Chungnam National University, Daejeon, 305-764, South Korea;(4) Department of Agricultural Chemistry, Sunchon National University, Sunchon, 540-742, Republic of Korea |
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Abstract: | Plant roots are associated with diverse communities of endophytic bacteria which do not exert adverse effects. The diversity
of bacterial endophytes associated with ginseng roots cultivated in three different areas in Korea was investigated. Sixty-three
colonies were isolated from the interior of ginseng roots. Phylogenetic analysis based on 16S rRNA gene sequences showed that
the isolates belonged to three major phylogenetic groups: the high G+C Gram-positive bacteria (HGCGPB), low G+C Gram-positive
bacteria (LGCGPB), and the Proteobacteria. The dominant species at the three different ginseng growing areas were: HGCGPB
at Ganghwa (55.0%), LGCGPB at Geumsan (45.5%), and Proteobacteria at Jinan (61.9%). Most cellulase-, xylanase-, and pectinase-producing
colonies among the isolates belong to the LGCGPB group, except for Pectobacterium carotovora which belonged to the Proteobacteria. The 13 isolates belonging to LGCGPB and Proteobacteria were assessed for their antifungal
activity against phytopathogenic fungi such as Rhizoctonia solani. Among them, Paenibacillus polymyxa GS01, Bacillus sp. GS07, and Pseudomonas poae JA01 show potential activity as biocontrol agents against phytopathogenic fungi. Finally, most of the low G+C Gram-positive
bacteria with antifungal activity against phytopathogenic microorganisms showed cellulolytic enzyme activity while some Proteobacteria
with the antifungal activity and the high G+C Gram-positive bacteria did not show any cellulolytic activity. |
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