A colorimetric method for detection and quantification of chlorinating activity of hemeperoxidases |
| |
Authors: | Manoj Kelath Murali Hager Lowell P |
| |
Institution: | Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. kmm@mail.wsu.edu |
| |
Abstract: | A method of detecting and assaying the halogenating activity of hemeperoxidases using the colored substrate, thionin, is reported here. In the presence of suitable amounts of peroxide and chloride, chloroperoxidase chlorinates thionin and bleaches the intense color of this substrate. The kinetics was quite similar to that of the established monochlorodimedone assay. Therefore, the thionin assay can be taken as an index of chlorinating activity. This reaction affords an escape from background problems and allows easy processing of large volumes of samples. |
| |
Keywords: | Chloroperoxidase Thionin Hemeperoxidase Chlorination assay |
本文献已被 ScienceDirect PubMed 等数据库收录! |