Oxygen concentration-dependent induction of a 140-kDa protein in magnetic bacterium Magnetospirillum magnetotacticum MS-1 |
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| Authors: | Hajime Sakaguchi Hiromi Hagiwara Yoshihiro Fukumori Yutaka Tamaura Minoru Funaki Shigehisa Hirose |
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| Institution: | Department of Biological Sciences, Tokyo Institute of Technology, Ookayama, Tokyo, Japan; Department of Life Science, Tokyo Institute of Technology, Ookayama, Tokyo, Japan; Department of Chemistry, Tokyo Institute of Technology, Ookayama, Tokyo, Japan; National Institute of Polar Research, Tokyo, Japan |
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| Abstract: | Abstract The magnetic bacterium Magnetospirillum magnetotacticum prefers a microaerobic habitat and should be able to sense oxygen. Therefore, the bacterium was cultured under atmospheres containing 0–5% O2 and analyzed for oxygen-dependent changes in the levels of its protein components by sodium dodecyl sulfate-polyccrylamide gel electrophoresis (SDS-PAGE). The analysis revealed a marked anaerobic induction of a 140-kDa protein, which was suppressed when M. magnetotacticum was switched from microaerobic (<1% O2) to aerobic (>1% O2) growth conditions. Although its function remains to be determined, the 140-kDa protein may serve as a useful tool to gain insight into the physiology of the organism. |
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| Keywords: | Magnetospirillum magnetotacticum Anaerobic induction Membrane protein |
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