| 解淀粉芽孢杆菌BamHI甲基转移酶基因克隆、功能鉴定与序列分析 |
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| 引用本文： | 刘洋,沈微,石贵阳,王正祥.解淀粉芽孢杆菌BamHI甲基转移酶基因克隆、功能鉴定与序列分析[J].生物技术通报,2009(11). |
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| 作者姓名： | 刘洋 沈微 石贵阳 王正祥 |
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| 作者单位： | 江南大学生物工程学院,工业生物技术教育部重点实验室,无锡,214122;江南大学生物工程学院生物资源与生物能源研究中心,无锡,214122 |
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| 基金项目： | 新世纪优秀人才支撑计划，国家"863"项目 |
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| 摘 要： | 从解淀粉芽孢杆菌Baillus amyloliquefaciens CICIM B2125中克隆了BamHI甲基转移酶基因(bamHIM),并在大肠杆菌JM109中得到了成功表达.该基因含有1 271 bp的开放阅读框(ORF),编码423个氨基酸,成熟蛋白分子量为49 kD.该基因在自身启动子引导下,表达了具有活性的BamHI甲基转移酶(M.BamHI).该酶可以将BamHI位点的碱基甲基化.氨基酸序列分析表明该酶存在有NADB_Rossmann结构域.
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| 关 键 词： | 解淀粉芽孢杆菌 BamHI甲基转移酶 序列分析 |
Cloning,Expression and Nucleotide Sequence Analysis of Gene Encoding for BamHI Methyltransferase from Baillus amyloliquefaciens CICIM B2125 |
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| Liu Yang,Shen Wei,Shi Guiyang,Wang Zhengxiang.Cloning,Expression and Nucleotide Sequence Analysis of Gene Encoding for BamHI Methyltransferase from Baillus amyloliquefaciens CICIM B2125[J].Biotechnology Bulletin,2009(11). |
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| Authors: | Liu Yang Shen Wei Shi Guiyang Wang Zhengxiang |
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| Abstract: | A gene encoding for BamHI methyltransferase(bamHIM )was cloned from Baillus amyloliquefaciens CICIM B2125. The bamHIM was expressed under its own promoter in E. coli JM109. The gene contained an opening reading frame ( ORF) of 1 271 bp, which coded for 423 amino acid residues. The molecular weight of mature protein was 49 kD. The BamHl site could be methylased by the enzyme M.BamHI. The amino acid sequence analysis revealed that the enzyme contained a domain of Rossmann-fold NAD(P) ( + ) -binding proteins. |
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| Keywords: | Baillus amyloliquefaciens BamHI methyltransferase Sequence analysis |
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