杉木远交亲本群体遗传多样性研究

借助ISSR分子标记技术，对杉木和侧柏远交亲本群体的遗传多样性进行了研究，采用国际上流行的软件（PopGENE）进行数据处理，获得如下结果：(1)群体多态性位点百分率为88.2%；(2)Nei’s基因多样性指数平均值为0.399 7;(3)Shannon信息指数多样性数为0.582 4;(4)群体内亲本间的平均遗传距离为0.669 8,变幅为(0.069 0,1.609 4),其变异系数为69.19%。经与国内外同类研究结果比较分析后认定:杉木远交亲本群体具有较高的遗传多样性,具有较大的改良潜力,由其衍生的生产群体亦具有较宽的生态适应性。

Genetic Diversity of Wide Cross Population of Cunninghamia lanceolata and Platycladu orientalis

By means of the technique of molecular marker of ISSR, genetic diversity of wide cross population of Chinese fir was studied, and the data collected in PCR experiment was processed by the worldwide popular software (PopGENE). The main results was as follows: (1)the effective number of alleles per locus was 1.719 1; (2)the percentage of polymorphic loci was 88.20%; (3)the Nei’s gene diversity index was 0.399 7; (4)Shannon’s information index was 0.582 4; (5) the genetic distance among parents within breeding population was(0.069 0, 1.609 4), the average value was 0.669 8, its PCV was 69.19%. Based on the comparison with similar study of the world, it can be confirmed that there was higher genetic diversity of parents population of Chinese fir and Platycladu orientalis. It possesses better improvement potential, and its corresponding productive population also had much wider ecological adaptability.