Vacuolar and cytoskeletal dynamics during elicitor-induced programmed cell death in tobacco BY-2 cells |
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| Authors: | Takumi Higaki Yasuhiro Kadota Tatsuaki Goh Teruyuki Hayashi Natsumaro Kutsuna Toshio Sano Seiichiro Hasezawa Kazuyuki Kuchitsu |
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| Affiliation: | 1.Department of Integrated Biosciences; Graduate School of Frontier Sciences; The University of Tokyo; Kashiwanoha Kashiwa, Chiba Japan;2.Department of Applied Biological Science; Tokyo University of Science; Noda, Chiba Japan;3.Genome & Drug Research Center; Tokyo University of Science; Noda, Chiba Japan;4.Institute for Bioinformatics Research and Development (BIRD); Japan Science and Technology Agency (JST) |
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| Abstract: | Responses of plant cells to environmental stresses often involve morphological changes, differentiation and redistribution of various organelles and cytoskeletal network. Tobacco BY-2 cells provide excellent model system for in vivo imaging of these intracellular events. Treatment of the cell cycle-synchronized BY-2 cells with a proteinaceous oomycete elicitor, cryptogein, induces highly synchronous programmed cell death (PCD) and provide a model system to characterize vacuolar and cytoskeletal dynamics during the PCD. Sequential observation revealed dynamic reorganization of the vacuole and actin microfilaments during the execution of the PCD. We further characterized the effects cryptogein on mitotic microtubule organization in cell cycle-synchronized cells. Cryptogein treatment at S phase inhibited formation of the preprophase band, a cortical microtubule band that predicts the cell division site. Cortical microtubules kept their random orientation till their disruption that gradually occurred during the execution of the PCD twelve hours after the cryptogein treatment. Possible molecular mechanisms and physiological roles of the dynamic behavior of the organelles and cytoskeletal network in the pathogenic signal-induced PCD are discussed.Key words: actin microfilament, cell cycle, cryptogein, microtubules, nuclei, programmed cell death, tobacco BY-2 cells, vacuoles |
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