基于UPLC-MS/MS定量检测法优化灵芝三萜热回流提取工艺的研究

基于超高效液相-三重四级杆质谱联用（UPLC-MS/MS）定量检测法优化出一种灵芝三萜热回流提取的工艺。通过本实验建立的灵芝三萜UPLC-MS/MS精确方法检测不同来源的7个栽培品种灵芝子实体中19个三萜化合物的含量,筛选出三萜含量较高的sd-2灵芝子实体作为提取原料,以三萜含量和提取物得率为指标,通过单因素和响应面实验对灵芝三萜的热回流工艺进行优化,获得的最佳条件为：乙醇浓度75%、提取时间2.5h、液料比14:1、提取次数2次。对获得的最佳提取工艺进行中试实验,灵芝子实体乙醇提取物的得率为6.10%,三萜含量为11.8591mg/g。研究结果可为灵芝三萜的工业大规模提取提供理论依据,为灵芝高附加值产品的开发利用提供技术支撑。

Optimization of the hot reflux extraction process of Ganoderma lingzhi triterpenoids based on UPLC-MS/MS quantitative detection method

Hot reflux extraction process was optimized for Ganoderma lingzhi triterpenoids based on ultra-high performance liquid-triple quadrupole mass spectrometry (UPLC-MS/MS) quantitative detection method. The established UPLC-MS/MS method was used to precisely detect the content of 19 triterpenoids of G. lingzhi fruiting bodies of seven cultivated varieties from different sources, and fruiting body of sd-2 G. lingzhi with high triterpenoids content was selected as the extractive material. Based on triterpenoid content and extract yield, the triterpenoid extraction process was optimized through single factor and response surface experiments. The best optimization conditions obtained were as follows: 75% of ethanol concentration, 2.5h of extraction time, 14:1 of liquid-to-material ratio, and two times of repeated extraction. A pilot experiment was conducted for obtained optimal extraction technology, and the yield of the ethanol extract of G. lingzhi fruiting body was 6.10%, and the content of triterpenoid was 11.8591mg/g. The results provide theoretical reference for the industrialized large-scale extraction of G. lingzhi triterpenoids and technical support of further development and utilization of G. lingzhi high value-added products.