高速逆流色谱法制备灵芝萜烯酮醇

本研究建立一种从灵芝子实体提取物中快速制备灵芝萜烯酮醇的方法。以沪农灵芝1号子实体为原料,经乙醇提取、D101大孔树脂富集后,再经一次正相色谱柱层析,获得富含灵芝萜烯酮醇的流分。采用高速逆流色谱法对该流分进行分离,优化分离条件,获得的最佳条件为：溶剂体系为正己烷-乙酸乙酯-甲醇-水（V/V/V/V,12:24:18:9）,流速2.0mL/min,转速900r/min,上样量500mg,一次上样可得到纯度为90.9%的灵芝萜烯酮醇52.1mg,得率达到10.4%。该方法具有操作简单、污染小、成本低、得率和纯度高的特点,是规模化制备灵芝萜烯酮醇的一种新方法。

Preparation of ganodermenonol by high speed countercurrent chromatography

A method to rapidly prepare ganodermenonol from the fruiting body extract of Ganoderma lingzhi was established. The fruiting body of G. lingzhi Hunong No.1 was used as raw material. After ethanol extraction, D101 macroporous resin enrichment, and one time normal phase column chromatography, the fraction rich in ganodermenonol was obtained. The fraction was separated by high-speed countercurrent chromatography, and the separation conditions were optimized. The best conditions obtained were: solvent system of hexane-ethyl acetate-methanol-water (V/V/V/V, 12:24:18:9), flow rate of 2.0mL/min, rotation speed of 900r/min, and loading quantity of 500mg. The ganodermenonol yield of 52.1mg per load could be obtained, with purity of 90.9% and yielding rate of 10.4%. This method is of simple operation, low pollution and cost, with obtained product of high yield and purity, and is a new method for large-scale preparation of ganodermenonol.