The proteome of the human neuroblastoma cell line SH-SY5Y: an enlarged proteome |
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| Authors: | Gilany Kambiz Van Elzen Roos Mous Kim Coen Edmond Van Dongen Walter Vandamme Stefaan Gevaert Kris Timmerman Evy Vandekerckhove Joël Dewilde Sylvia Van Ostade Xaveer Moens Luc |
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| Institution: | Department of Biomedical Sciences, Protein Chemistery, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. |
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| Abstract: | The human neuroblastoma cell line SH-SY5Y (ATCC: CRL-2266) is widely used as a neural cellular model system. The hitherto existing proteome data (115 proteins) are here extended. A total of 1103 unique proteins of this cell line were identified using 2D-LC combined with MALDI-TOF/TOF-MS, SDS-PAGE with nano-LC-MS/MS, N-terminal COFRADIC analysis with nano-LC-MS/MS and 2D-PAGE with MALDI-TOF/TOF-MS peptide mass fingerprinting. The obtained proteome profile of this cell line is discussed. |
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