Metabolic studies on retinoic acid in the rat |
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Authors: | P. R. Sundaresan and H. N. Bhagavan |
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Affiliation: | Lipids Laboratory, Research Institute, St Joseph Hospital, Lancaster, Pa. 17604, U.S.A. |
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Abstract: | The nature of metabolites in the urine arising from differentially labelled retinoic acid was investigated after injection of physiological doses into retinol-deficient rats. Distribution of radioactivity after partition of urine into ether-soluble, acidic and water-soluble fractions revealed that there were at least six metabolites in urine. Of these, the major metabolite(s) was one lacking both C-14 and C-15 of retinoic acid. Enzymic or alkaline hydrolysis of acidic and water-soluble fractions did not release any retinoic acid, thus indicating that retinoyl beta-glucuronide was not present in urine in significant amounts. |
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