A switchable light-input, light-output system modelled and constructed in yeast |
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| Authors: | Oxana Sorokina Anita Kapus Kata Terecskei Laura E Dixon Laszlo Kozma-Bognar Ferenc Nagy Andrew J Millar |
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| Institution: | (1) Institute of Molecular Plant Sciences, The University of Edinburgh, Kings Buildings, Mayfield Road, Edinburgh, EH9 3JH, UK;(2) Institute of Plant Biology, Biological Research Center, Temesvari krt. 62, Szeged, H-6726, Hungary;(3) Centre for Systems Biology at Edinburgh, C.H. Waddington Building, Kings Buildings, Mayfield Road, Edinburgh, EH9 3JD, UK |
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| Abstract: | Background Advances in synthetic biology will require spatio-temporal regulation of biological processes in heterologous host cells.
We develop a light-switchable, two-hybrid interaction in yeast, based upon the Arabidopsis proteins PHYTOCHROME A and FAR-RED
ELONGATED HYPOCOTYL 1-LIKE. Light input to this regulatory module allows dynamic control of a light-emitting LUCIFERASE reporter
gene, which we detect by real-time imaging of yeast colonies on solid media. |
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