| 金丝小枣多糖ZP3c的分离纯化及其组成分析 |
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| 引用本文： | 李进伟,范柳萍,丁霄霖,张连富.金丝小枣多糖ZP3c的分离纯化及其组成分析[J].天然产物研究与开发,2009,21(6):984-987. |
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| 作者姓名： | 李进伟 范柳萍 丁霄霖 张连富 |
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| 作者单位： | 江南大学食品学院食品科学与技术国家重点实验室,无锡,214122 |
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| 基金项目： | 江南大学食品学院青年博士科研创新开放基金 |
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| 摘 要： | 经DEAE-SepharoseCL-6B、SepharoseCL-6B和Sephadex G-200柱层析,得到一个均一的金丝小枣多糖组分(ZP3c).ZP3c的中性单糖是由鼠李糖、阿拉伯糖和半乳糖组成,其摩尔比为1:2.13:19.3.经红外光谱测定,ZP3c的酯化度为41.85%.
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| 关 键 词： | 金丝小枣 多糖 分离纯化 组成 |
Isolation,Purification and Composition of Polysaccharide ZP3c from Zizyphus jujuba cv.Jinsixiaozao |
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| LI Jin-wei,FAN Liu-ping,DING Xiao-lin,ZHANG Lian-fu.Isolation,Purification and Composition of Polysaccharide ZP3c from Zizyphus jujuba cv.Jinsixiaozao[J].Natural Product Research and Development,2009,21(6):984-987. |
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| Authors: | LI Jin-wei FAN Liu-ping DING Xiao-lin ZHANG Lian-fu |
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| Abstract: | a water-soluble polysaccharide(ZP3c)was purified by DEAE-SephatoseCL-6B,Sepharose CL-6B and Sephadex G-200 column chromatography.The purity of ZP3c was verified by high-performance liquid chromatography (HPLC),specific rotatory power teat and agaroae gel eleetrophoresis.The average molecular weight WaS estimated to be 1.6×105 Da.GC analysis result showed that ZP3c was composed mainly of rhamnose,arabinose and galactose in a molar ratio of 1:2.13:19.3.The degree of esterifieafion(DE)value of ZP3c was 41.85% by Fourier transform infrared spectrophotometer. |
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| Keywords: | Zizyphus jujuba cv Jinsixiaozao polysaccharide isolation and purification composition |
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