| 蛇床幼茎离体培养中体细胞胚胎形成的观察 |
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| 引用本文： | 郝建平,周小梅,李宝平,郭双生,陈柔如,张江涛.蛇床幼茎离体培养中体细胞胚胎形成的观察[J].武汉植物学研究,1994,12(3):247-250. |
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| 作者姓名： | 郝建平 周小梅 李宝平 郭双生 陈柔如 张江涛 |
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| 作者单位： | 山西大学生物系，山西运城高等专科学校，北京中国科学院植物研究所 |
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| 摘 要： | 蛇床幼茎外植体经诱导产生了愈伤组织。在ＭＳ＋２，４－Ｄ，０．２ｍｇ／Ｌ＋ＺＴ０．４ｍｇ／Ｌ培养基中，愈伤组织转变成胚性愈伤组织。转入ＭＳ＋ＮＡＡ０．２ｍｇ／Ｌ＋ＺＴ０．８ｍｇ／Ｌ培养基以后，胚性愈伤组织分化出体细胞胚胎。体细胞胚胎在ＭＳ＋ＮＡＡ０．５ｍｇ／Ｌ培养基中可直接发育成为完整植析。显微观察表明，体细胞胚胎产生于愈伤组织的表层细胞或内部细胞。在鱼雷胚期已有螺纹导管的分化。子叶期的维管组织从两
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| 关 键 词： | 蛇床 组织培养 胚状体 体细胞胚胎发生 |
| 收稿时间： | 1992-10-1 |
| 修稿时间： | 1993-1-4 |
OBSERVATIONS ON SOMATIC EMBRYOGENESIS OF CNIDIUM MONNIERI CULTURED IN VITRO |
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| Hao Jianping,Zhou Xiaomei,Li Baoping,Guo Shuangsheng,Chen Rouru,Zhang Jiangtao.OBSERVATIONS ON SOMATIC EMBRYOGENESIS OF CNIDIUM MONNIERI CULTURED IN VITRO[J].Journal of Wuhan Botanical Research,1994,12(3):247-250. |
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| Authors: | Hao Jianping Zhou Xiaomei Li Baoping Guo Shuangsheng Chen Rouru Zhang Jiangtao |
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| Institution: | Department of Biology,Shanxi University Taiyuan 030006 |
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| Abstract: | Light yellow-green calli were formed from young stem explants of Cnidium monnieri cultured on MS medium containing 2mg/L 2,4-D,0.2mg/L KT and 1000mg/LH.The calli were cultured on MS medi-um supplemented with 0.2mg/L2,4-D,0.4mg/L ZT and 1000mg/LCH,embryogenic calli were obtained.On MS medium supplemented with 0.2mg/L NAA and 0.8mg/L ZT, prolonged culture of the embryogenic calli resulted in embryoids formation.Germination of the embryoids into plantlets was achieved on MS medium added 0.5mg/L NAA.Section of speciments for microscopy showed that the embryoids were originated from the periphery or the interior of embryogenic calli. |
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| Keywords: | Cnidium monnieri Tissue culture Embryoid Somatic embryogenesis |
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