无创性皮肤屏障功能检测在朗格汉斯细胞组织细胞增生症中的应用

摘要 目的：探讨无创性皮肤屏障功能检测在朗格汉斯细胞组织细胞增生症(Langerhans cell histiocytosis，LCH)中的应用价值。方法：研究时间为2017年1月到2020年12月，选择在本院诊治的朗格汉斯细胞组织细胞增多症患者72例作为LCH组，同期选择健康体检者72例作为对照组。采用无创性皮肤屏障功能检测皮肤水分、经皮水分丢失(Transdermal water loss，TEWL)、油脂水平，同时检测所有入选者的免疫功能、皮肤菌群并进行相关性分析。结果：LCH组的皮肤水分低于对照组(P<0.05)，TEWL、油脂水平高于对照组(P<0.05)。LCH组的乳酸杆菌(La)阳性率低于对照组(P<0.05)，表皮葡萄球菌(Se)、痤疮丙酸杆菌(Pa)、金黄色葡萄球菌(Sa)阳性率高于对照组(P<0.05)。LCH组的CD163、ki-67表达阳性率分别为77.8 %、52.8 %，高于对照组的19.4 %和6.9 %(P<0.05)。在LCH组中，Pearson相关性分析显示皮肤水分与乳酸杆菌呈现正相关性(P<0.05)，TEWL、油脂与表皮葡萄球菌、痤疮丙酸杆菌、金黄色葡萄球菌、CD163、ki-67呈现正相关性(P<0.05)。结论：无创性皮肤屏障功能检测在朗格汉斯细胞组织细胞增生症中的应用可反映患者的皮肤水分与油脂状况，也可间接反映患者的皮肤微生态与免疫功能状况。

Application of Non-invasive Skin Barrier Function Detection in Langerhans Cell Histiocytosis

ABSTRACT Objective: To investigate the application values of non-invasive skin barrier function detection in Langerhans cell histiocytosis (LCH). Methods: From January 2017 to December 2020,72 cases of patients with Langerhans cell histiocytosis treated in our hospital were selected as the LCH group, and the other 72 cases of health examination were selected as the control group during the same period. The non-invasive skin barrier function were used to detect skin moisture, transdermal water loss (TEWL) and oil levels. At the same time, the immune function and skin flora of all caseswere tested and given correlation analysis. Results: The skin moisture of the LCH group were lower than that of the control group (P<0.05), and the TEWL and oil levels were higher than that of the control group (P<0.05). The positive rates of Lactobacillus (La) in the LCH group were lower than that in the control group (P<0.05), and the positive rates of Staphylococcus epidermidis (Se), Propionibacterium acnes (Pa), and Staphylococcus aureus (Sa) were higher than those in the control group (P<0.05). The positive rates of CD163 and ki-67 expression in the LCH group were 77.8 % and 52.8 %, respectively, which were higher than 19.4 % and 6.9 % in the control group (P<0.05). In the LCH group, Pearson correlation analysis showed there were positive correlation between skin moisture and Lactobacillus(P<0.05), TEWL, oil were positive correlated to the Staphylococcus epidermidis, Propionibacterium acnes, Staphylococcus aureu(P<0.05). Conclusion: The application of non-invasive skin barrier function detection in Langerhans cell histiocytosis can reflect the patient''s skin moisture and oil status, and can also indirectly reflect the patient''s skin microecology and immune function status.