炙甘草汤加减缓解神经根型颈椎病大鼠疼痛和对炎症反应的影响及机制研究

摘要 目的：探究炙甘草汤加减缓解神经根型颈椎病大鼠疼痛和对炎症反应的影响及机制。方法：采用免疫组织化学对接受炙甘草汤加减治疗的大鼠的脊髓组织神经元、小胶质细胞和星形胶质细胞中sPLA2的表达进行检测。使用免疫组织化学法通过测量DNA损伤标记物8-OHG检测氧化应激的程度。结果：与在神经根受压之前进行炙甘草汤加减灌胃可显著减少脊髓炎症以及DRG中的外周氧化损伤（P<0.05）。炙甘草汤加减降低了脊髓中的小胶质细胞和星形胶质细胞的激活，差异有统计学意义（P<0.05）。与第7天神经胶质激活减少的同时，脊髓sPLA2的产生亦受到抑制，神经胶质和神经元均减少，差异有统计学意义（P<0.05）。在疼痛性神经根损伤后，氧化应激标记物8-OHG几乎只存在于脊髓神经元中。在神经创伤前立即进行炙甘草汤加减治疗可防止外周DRG神经元中DNA和RNA中8-OHG增加，差异有统计学意义（P<0.05）。结论：炙甘草汤加减可以通过减少中枢和外周神经炎症和氧化应激来预防疼痛的发展。

Study on the Effect and Mechanism of the Addition and Subtraction of Zhigancao Decoction on Alleviating Pain and Inflammation in Rats with Cervical Spondylotic Radiculopathy

ABSTRACT Objective: Exploring the effect and mechanism of Zhigancao Decoction on alleviating pain and inflammation in rats with cervical spondylotic radiculopathy. Methods: Immunohistochemistry was used to detect the expression of sPLA2 in spinal cord tissue neurons, microglia and astrocytes of rats that received the addition and subtraction of Zhigancao Decoction. Immunohistochemistry was used to detect the degree of oxidative stress by measuring the DNA damage marker 8-OHG. Results: The addition and subtraction of Zhigancao Decoction before nerve root compression can significantly reduce spinal cord inflammation and peripheral oxidative damage in DRG (P<0.05). The addition and subtraction of Zhigancao Decoction decreased the activation of microglia and astrocytes in spinal cord, and the difference was statistically significant (P<0.05). At the same time as the decrease of glial activation on the 7th day, the production of sPLA2 in spinal cord was also inhibited, and the glia and neurons were reduced, the difference was statistically significant (P<0.05). After painful nerve root injury, the oxidative stress marker 8-OHG exists almost only in spinal cord neurons. In addition to pain and spinal cord inflammation, the addition and subtraction of Zhigancao Decoction treatment before nerve trauma could prevent the increase of 8-OHG in DNA and RNA in peripheral DRG neurons (P<0.05). Conclusion: The addition and subtraction of Zhigancao Decoction can prevent the development of pain by reducing the inflammation and oxidative stress of the central and peripheral nerves.