Isolation and characterization of glycoprotein lectins from the bark of three species of elder,Sambucus ebulus,S. nigra and S. racemosa |
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Authors: | M Nsimba-Lubaki W J Peumans A K Allen |
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Institution: | (1) Laboratorium voor Plantenbiochemie, Katholieke Universiteit Leuven, Kardinaal Mercierlaan 92, B-3030 Leuven (Heverlee), Belgium;(2) Department of Biochemistry, Charing Cross and Westminster Medical School, Hammersmith, W6 8RF London, UK |
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Abstract: | Lectins have been isolated from the bark of three members of the family Caprifoliaceae, Sambucus nigra (elder), S. racemosa (red-berried elder) and S. ebulus (dwarf elder), by affinity chromatography on fetuin-agarose, ion-exchange and gel-filtration chromatography. They are all glycoproteins of M
r 140 000 made up of at least four subunits. The lectin have similar but not identical amino-acid compositions and the carbohydrate content varies between 12% and 19% (w/w), the main sugars being (N-acetyl)glucosamine, mannose, fucose and xylose. Inhibition studies of hemagglutination with various mono- and oligosaccharides have shown that N-acetylgalactosamine and galactose together with galactose-containing oligosaccharides are the most effective inhibitors. There are some differences in specificity, in particular S. ebulus agglutinin is inhibited to the same degree by galactosamine, N-acetylgalactosamine and by galactose.Abbreviations PBS
phosphate-buffered saline
- SDS-PAGE
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
- SEA
S. ebulus agglutinin
- SNA
S. nigra agglutinin
- SRA
S. racemosa agglutinin |
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Keywords: | Bark (lectin) Glycoprotein Lectin Sambucus |
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