外周血CD14~(high)CD16~+单核细胞在HIV/HCV重叠感染者肝损伤中的作用

目的:探讨HIV/HCV重叠感染患者外周血单核细胞亚群与肝损伤的关系。方法:观察对象为HIV/HCV重叠感染患者,分为对照组(n=11)、肝纤维化组(n=12)和肝硬化组(n=7)。运用流式细胞仪检测单核细胞及其亚群变化,瞬时弹性成像(Fibroscan)检测肝纤维化情况。比较单核细胞各亚群在不同程度肝损伤中的差异,并对HIV/HCV重叠感染患者外周血的单核细胞数与肝纤维化情况进行相关性分析。结果:HIV/HCV重叠感染患者肝硬化组与对照组比较,单核细胞CD14low CD16+和CD14high CD16+亚群显著增多(P=0.047,P=0.018)。HIV/HCV重叠感染患者肝纤维化组与对照组比较,单核细胞各亚群差异无统计学意义(P=0.84,P=0.812)。HIV/HCV重叠感染患者CD14high CD16+单核细胞与肝纤维化情况存在正性线性相关,方程成立,并且系数有统计学意义(P=0.018),方程似然比(r 2)0.45。结论:HIV/HCV重叠感染患者CD14high CD16+单核细胞增高有可能是肝损伤加重的原因之一。

Role of CD14highCD16+Mononuclear Cells in Peripheral Blood of Liver Injured Patients with Human Immunodeficiency Virus and Hepatitis C co-infection

Objective:To investigate the role of CD14high CD16+ monocytes in peripheral blood of liver damage progression in co-infected with HIV/HCV.Methods:The HIV/HCV co-infected patients were divided into three groups, control group, and liver cirrhosis group. Flow cytometry was used to examine mononuclear cells and subpopulations, and transient elastography (Fibroscan) was used to detect the liver fibrosis. Difference monocytes subsets in three groups with different degree of liver injury in patients with HIV/HCV co-infection were compared, and the correlation between the mononuclear cells subpopulations and liver fibrosis analyzed.Results:The percentage of CD14low CD16+ and CD14high CD16+mononuclear cells in liver cirrhosis group with HIV/HCV co-infection was in- creased significantly compared with the control group in patients with HIV/HCV co-infection (P= 0.047, P=0.018). There was no differ- ence of mononuclear cells and subsets in the hepatic fibrosis group and the control group of overlapping infection of HIV/HCV (P=0.84, P=0. 812). There was positive linear correlation between CD14high CD16+ monocytes percentage and liver injury (r 2=0.45).Conclusion:CD14high CD16+monocytes may play a pivotal role in aggravating liver injury of patients with HIV/HCV co-infection.