Metabarcoding of PalEnDNA as An Efficient Tool to Recover Ancient Bacterial Diversity |
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| Authors: | Shan P. Thomas Bhavatharini Shanmuganathan Sivakumar Krishnan Kartika Goswami Maha Dev Manoj Kumar Jaiswal |
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| Affiliation: | 1. Department of Biotechnology, National College (Autonomous), Tiruchirappalli, India;2. Department of Geology, National College (Autonomous), Tiruchirappalli, India;3. Department of Earth Sciences, Indian Institute of Science and Education Research (IISER), Kolkata, India |
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| Abstract: | Past bacterial diversity of a paleosol was reconstructed using metabarcoding of paleo environmental DNA (PalEnDNA). The paleosol was subsampled from a sediment core which was excavated from a palaeo beach-ridge located 2.6?km away from present sea shore and identified that it was deposited under marine influence ～6000?years ago, using geological proxies. The bacterial community contained 37 bacterial phyla and dominated by Proteobacteria, followed by Bacteroidetes, Firmicutes, Actinobacteria, Verrucomicrobia, and Chloroflexi. The bacterial community was a mix-up of marine and terrestrial population, and thereby diversity was higher than marine populations. The result shows metabarcoding of PalEnDNA can effectively reconstruct past bacterial community structure. |
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| Keywords: | Bacterial community illumina metabarcoding PalEnDNA |
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