| 分散蓝102标准品的制备研究 |
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| 引用本文： | 郭卫荣,李懿睿.分散蓝102标准品的制备研究[J].氨基酸和生物资源,2009,31(2):81-83. |
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| 作者姓名： | 郭卫荣 李懿睿 |
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| 作者单位： | 上海市计量测试技术研究院,上海,201203;上海市计量测试技术研究院,上海,201203 |
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| 摘 要： | 将商品化的分散蓝102以二甲基甲酰胺溶解,运用制备高效液相色谱制备分散蓝102标准品,纯度大于99%。制备型色谱柱为Waters Xbridge C18柱(5μm,30 mm×150 mm);流动相为10 mmol.L-1碳酸氢铵水溶液和乙腈;流速为30 ml.min-1;检测波长为254 nm;柱温为室温。该方法所得分散蓝102纯度高,可用作分析方法的对照品。
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| 关 键 词： | 制备高效液相色谱法 分散蓝102 标准品 |
Study on Preparation of Standard Substance Disperse Blue 102 |
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| Guo Wei-rong,Li Yi-rui.Study on Preparation of Standard Substance Disperse Blue 102[J].Amino Acids & Biotic Resources,2009,31(2):81-83. |
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| Authors: | Guo Wei-rong Li Yi-rui |
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| Institution: | Guo Wei - rong, Li Yi - rui( Shanghai Institute of Mesurement and Testing Technology, Shanghai 201203, China) |
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| Abstract: | After the Disperse Blue 102 on sale was dissolved in DMF,it was separated and purified by preparative high performace liquid chromatograph.The purity of Disperse Blue 102 was over 99%.Preparative chromatographic column Waters Xbridge C18(5μm,30mm×150mm)was used at room temperature with acetonitrile-10mmoL·L-1ammonium acid carbonate at a flow rate of 30 mL·min-1.UV detection wavelength was set at 254 nm.The purity of Disperse Blue 102 by this method was high and could be used as a reference substance. |
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| Keywords: | preparative high performance liguid chromatograph disperse Blue 102 standard substance |
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