| 共聚焦显微技术研究ABA诱导蚕豆气孔保卫细胞H_2O_2的产生(简报) |
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| 引用本文： | 张骁,张霖,安国勇,高俊凤,宋纯鹏.共聚焦显微技术研究ABA诱导蚕豆气孔保卫细胞H_2O_2的产生(简报)[J].分子细胞生物学报,2001(1). |
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| 作者姓名： | 张骁 张霖 安国勇 高俊凤 宋纯鹏 |
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| 作者单位： | 西北农林科技大学生命科学学院，河南大学生物学系，河南大学生物学系，西北农林科技大学生命科学学院，河南大学生物学系 陕西杨陵712100 河南大学生物学系，开封 475001，开封 475001，陕西杨陵712100，开封 475001 |
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| 基金项目： | 国家自然科学基金(No.39870372)，河南省自然科学基金(No.994012300)~~ |
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| 摘 要： | 逆境下,植物细胞内ABA含量急剧增加,同时植物也可通过一些酶代谢反应积累活性氧,如H_2O_2,O_2~-。ABA作为逆境信号对气孔运动的显著调节作用已被诸多实验所证实,但关于其对气孔运动调节的细节还知之甚少。H_2O_2作为氧化信号分子在植物抗病信号转导中已得到广泛研究,但H_2O_2是否介导保卫细胞的气孔运动还缺乏直接的证据。我们已初步发现H_2O_2可参与外源ABA诱
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| 关 键 词： | ABA H_2O_2 蚕豆 显微注射 激光共聚焦显微术 |
STUDIES ON ABA-INDUCED H_2O_2 IN Vicia GUARD CELLS BY MEANS OF CONFOCAL LASER SCANNING MICROSCOPY |
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| ZHANG Xiao ZHANG Lin AN Guo Yong GAO Jun Feng SONG Chun Peng.STUDIES ON ABA-INDUCED H_2O_2 IN Vicia GUARD CELLS BY MEANS OF CONFOCAL LASER SCANNING MICROSCOPY[J].Journal of Molecular Cell Biology,2001(1). |
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| Authors: | ZHANG Xiao ZHANG Lin AN Guo Yong GAO Jun Feng SONG Chun Peng |
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| Abstract: | The methods of confocal laser scanning microscopy (CLSM) and microinjection were used to study ABA-induced H2O2 in guard cells ( Vicia faba ), which were labeled with H2O2 specific probe-2, 7-dichlorofluorescin diacetate(H2DCFDA). The results indicated 100U/mL catalase (CAT) could inhibit partly stomatal clasure induced by ABA. 10-3mmol/L ABA could significantly
induce H2O2 production in chloroplasl in guard cells of Vicia faba following microinjection, and 100U/mL CAT could partly abolish the effects following simultaneous microinjection of ABA and CAT. These suggest that H2O2 is possibly involved in ABA signaling leading to stomatal closure. |
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| Keywords: | ABA H_2O_2 Vicia faba Microinjection Confocal laser scanning microscopy |
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