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Mitochondrial cytochrome components of Paragonimus adult worms
Authors:K Yamakami  T Oguma  F Hamajima  K Fukuda
Institution:

Department of Parasitology, National Defense Medical College, 359, Tokorozawa, Japan

Abstract:The cytochrome components of adult Paragonimus miyazakii mitochondria were investigated by polyacrylamide gel electrophoresis. The mitochondria were found to contain cytochromes b, c1, c and aa3. Two types of mitochondria, lightweight mitochondria (LWMt) and heavyweight mitochondria (HWMt), were obtained by centrifugation from the mitochondrial fraction of the adult Paragonimus ohirai. The succinate-reduced and oxidized difference spectrum of LWMt and HWMt at ?196°C revealed that both mitochondria contained at least functional levels of cytochromes b, c1, c and a low value of aa3. Although succinate-reduced cytochromes of LWMt reoxidized in the presence of air, those of HWMt did so only minimally.
Keywords:Paragonimus miyazakii  Paragonimus ohirai  lightweight mitochondria  heavyweight mitochondria  polyacrylamide gel electrophoresis  cytochrome b  cytochrome c1  cytochrome c  cytochrome aa3  difference spectrum
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