茉莉酸甲酯抑制拟南芥根伸长生长电生理学机制

以外源茉莉酸甲酯(JA-Me)处理拟南芥,运用膜片钳技术研究JA-Me、过氧化氢(H2O2)和内向K+通道之间的关系,以探讨茉莉酸类物质(JAs)抑制根伸长生长分子机制。检测到10-4mol/L的JA-Me能抑制根细胞质膜内向K+电流,表明可能与根的伸长生长有关,并且发现H2O2可能作为第二信使参与了JAs抑制根伸长生长的过程,H2O2介导的JA-Me对根细胞内向K+通道的抑制是根生长受抑的可能电生理机制。

Electrophysiological mechanism of root length growth of Arabidopsis thaliana inhibition by JA-Me

Arabidopsis thaliana(ecotype Columbia)was treated with methyl-jasmonate(JA-Me)and the relations of JA-Me,H2O2 and inward potassium channel were examined by using patch clamp technique.The results showed that different concentrations of JA-Me(10-8-10-3mol/L)could all inhibit the elongation of primary roots to some degree.The inward K+ currents of root cortical protoplasts were inhibited with 10-4 mol/L JA-Me and 10-5 mol/L H2O2 treatment.What's more,10-4 mol/L DPI and 10-2 mol/L Vc could both reverse the JA-Me-inhibited inward K+ currents respectively,which suggested that H2O2 may act as a second messenger involving JA-Me inhibition of the root inward K+ currents.The results indicate that JA-Me inhibition of root length growth may result from the H2O2 induced JA-Me inhibition of the root inward potassium channel.