利用RAPD法对虹鳟鱼基因组DNA多态性研究初报

本文通过利用RAPD（随机扩增多态DNA）方法，选用20种10bp的随机引物（OPC－01－OPC—20），用于虹鳟鱼基因组DNA多态性分析，其中发现一种引物（OPC－02）在虹鳟鱼群体中能扩增出多条带，说明该引物能反映其基因组DNA的多态性，可用于检测出不同品系间的差异。

The tentative study on rainbow trout genomic DNA polymorphisms applied RAPD assay

This paper applies RAPD (Random Ampified Polymorphic DNA) assay to detect rainbow trout genomic DNA polymorphismw. We chose 20 different kinds of oligonnucleotide 10-mer primers (OPC01 -OPC20) and found that only a primer cound amplify 0- 3 bands in mixed rainbow trout groups: It says this primer can show the genomic DNA porlymorphisms and can use. to detect genomic differences in different strains of rainbow trout.