| 未知基因P2.18 |
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| 引用本文： | 张永亮,欧阳红生.未知基因P2.18[J].生物技术,1998,8(1):7-9. |
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| 作者姓名： | 张永亮 欧阳红生 |
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| 作者单位： | 长春农牧大学生化教研室 长春130062
(张永亮，欧阳红生，冯立文，岳军明，刘松财)，长春农牧大学生化教研室 长春130062(张玉静) |
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| 摘 要： | 用根据GRF设计的引物,以猪的下丘脑总RNA和mRNA为模板、进行RT—PCR扩增.将扩增产物纯化并克隆到T载体(pGEM3—Zf),自动测序仪测定其序列.发现所克隆的基因并非GRF基因.应用BLAST软件对其在INTERNET作了分析,未能确定为何种基因.
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| 关 键 词： | GRF RT-PCR扩增 T载体 |
Unknown Gene P2.18 |
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| Zhang Yongliang Ouyang Hongshang Feng Liweng Junming Lui Songcai Zhang Yujing.Unknown Gene P2.18[J].Biotechnology,1998,8(1):7-9. |
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| Authors: | Zhang Yongliang Ouyang Hongshang Feng Liweng Junming Lui Songcai Zhang Yujing |
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| Abstract: | We designed a experiment to obtain porcine GRF cDNA. Total RNA and mRNA were extracted from porcine hypotheiemus. Primers were designed according to publised porcine GRF cDNA sequence. RT-PCR was carried out to the standard method. PCRproduct was cloned to T-vector. Positive clone was selected and sequenced. The result showed that the cloned cDNA was not GRF gene. We anylyzed the sequence in Internet by Blast, finding no similar. |
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| Keywords: | RT-PCR GRF PGEM3-Zf |
| 本文献已被 CNKI 维普 等数据库收录！ |
