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Control of Dioscorea alata microtuber dormancy and germination by jasmonic acid
Authors:Romain Bazabakana  Marie-Laure Fauconnier  Billo Diallo  Jean Pierre Dupont  Jacques Homes  Mondher Jaziri
Affiliation:(1) Laboratory of Plant Biotechnology, Université Libre de Bruxelles, Chaussée de Wavre, 1850, B-1160 Bruxelles, Belgium;(2) Mondher JAZIRI: Phone, Belgium
Abstract:Effects of appling exogenous jasmonic acid (JA) on the germination of Dioscorea alata L. microtubers were examined on Murashige and Skoog (MS) medium. Microtuber germination was promoted by JA (0.1 and 1 mgrM) supplemented to the culture medium but higher concentrations (30 and 100 mgrM) completely inhibited germination. When these inhibited microtubers were transferred to hormone-free medium, germination resumed.After transfer to greenhouse conditions, almost all plants (95%) from tubers previously cultivated on MS medium with 100 mgrM JA survived and all acclimatized plants had produced tubers after 8 months. It is concluded that depending on JA concentration, both the germination and dormancy processes in D. alata microtubers were affected. The release from dormancy is easily obtained by transferring dormant microtubers to hormone-free medium.
Keywords:dormancy  germination  jasmonic acid  microtubers  storage  yam
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