Cloning type-II restriction and modification genes |
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| Affiliation: | 1. Biomedical Engineering and the Center for Biological Systems Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA;2. Division of Biology and Biomedical Sciences, Washington University in St. Louis, St. Louis, MO 63130, USA;1. AP-HP, Internal Medicine Department 2, Groupe Hospitalier Pitié Salpêtrière, 75013 Paris, France;2. AP-HP, Internal Medicine Department, Hôpital Saint-Antoine, 75012 Paris, France;3. AP-HP, Immunochemistry Laboratory, Groupe Hospitalier Pitié Salpêtrière, 75013 Paris, France;4. Internal Medicine Department, Hôpital Claude Huriez, CHRU, 59000 Lille, France;5. University Hospital, Lille, 75013 Paris, France;6. Université Pierre et Marie Curie, Paris 6, 75013 Paris, France;7. Versailles-Saint-Quentin-en-Yvelines University, 78000 Versailles, France;8. AP-HP, Service de Dermatologie-Allergologie, Hôpital Tenon, 75020 Paris, France;1. Department of Sciences, Roma Tre University, Viale Guglielmo Marconi 446, 00146 Roma, Italy;2. Technical Scientific Service and Core Facilities, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy;3. Department of Clinical Sciences and Translational Medicine, University of Roma “Tor Vergata”, Via Montpellier 1, I-00133 Roma, Italy;4. Centro de Investigatiòns Cientìficas Avanzadas (CIA), Departamento de Quìmica, Facultade de Ciencias, Universidad da Coruña, 15071 A Coruña, Galicia, Spain;5. Department of Physics, University of Genova, Via Dodecaneso 33, 16100 Genova, Italy;6. Interdepartmental Laboratory of Electron Microscopy, Roma Tre University, Via della Vasca Navale 79, I-00146 Roma, Italy;1. Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada;2. Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada;3. Integrated Oncology Program, British Columbia Cancer Agency, Vancouver, British Columbia, Canada;4. Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada;5. Department of Pathology and Laboratory Medicine, British Columbia Cancer Agency, Vancouver, British Columbia, Canada;6. Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada |
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| Abstract: | We have cloned into Escherichia coli the genes for 38 type-II bacterial modification methyltransferases. The clones were isolated by selecting in vitro for protectively modified recombinants. Most of the clones modify their DNA fully but a substantial number modify only partially. In approximately one-half of the clones, the genes for the corresponding endonucleases are also present. Some of these clones restrict infecting phages and others do not. Clones carrying endonuclease genes but lacking methyltransferase genes have been found, in several instances, to be viable. |
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