| 外源EGFP基因在肿瘤细胞中的稳定表达 |
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| 引用本文： | 亓毅飞,谭卓卓,黄嘉琪.外源EGFP基因在肿瘤细胞中的稳定表达[J].生物学杂志,2011,28(4):10-13,17. |
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| 作者姓名： | 亓毅飞 谭卓卓 黄嘉琪 |
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| 作者单位： | 中山大学药学院实验中心,广州,510006 |
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| 摘 要： | 通过将增强型绿色荧光蛋白（EGFP）基因转染到人肺腺癌细胞（A549）内,建立以EGFP为探针的体外抗癌药物细胞株。使用电穿孔法导入EGFP基因到A549细胞;然后用G418筛选以及梯度稀释法筛选出EGFP高度表达的细胞株。初步建立稳定表达绿色荧光蛋白的人肺癌细胞株。
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| 关 键 词： | 肺腺癌A549细胞 增强型绿色荧光蛋白 电穿孔 |
EGFP of extraneous genes stable-expressed in tumor cells |
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| QI Yi-fei,TAN Zhuo-zhuo,HUANG Jia-qi.EGFP of extraneous genes stable-expressed in tumor cells[J].Journal of Biology,2011,28(4):10-13,17. |
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| Authors: | QI Yi-fei TAN Zhuo-zhuo HUANG Jia-qi |
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| Institution: | QI Yi-fei,TAN Zhuo-zhuo,HUANG Jia-qi(School of Pharmaceutical Sciences,Sun Yet-Sen University,Guangzhou 510006,China) |
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| Abstract: | The enhanced green fluorescent protein(EGFP) gene was transfected into the chromosomes of the human lung adenocarcinoma cells(A549) to construct the cell model with the EGFP for anti-cancer drugs screening in vitro,The application value of the model through studying in vivo as well as in vitro was studied.By electroporation,the EGFP gene was transfected into A549 cells and then by means of the G418 and gradient method,the high EGFP-expression cells that cultured for expansion were filtered out.The anti-canc... |
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| Keywords: | A549 cells EGFP the strategy of electroporation |
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