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| 大肠杆菌DH5α菌株感受态制备及转化率变化研究 | |
| 引用本文： | 谢翎,陈红梅,尹翀,项小燕,黄勃.大肠杆菌DH5α菌株感受态制备及转化率变化研究[J].生物学杂志,2011,28(4):4-6. |
| 作者姓名： | 谢翎 陈红梅 尹翀 项小燕 黄勃 |
| 作者单位： | 1. 安庆师范学院生命科学学院,安庆,246011;安徽农业大学微生物防治省重点实验室,合肥,230036 2. 安庆师范学院生命科学学院,安庆,246011 3. 安徽农业大学微生物防治省重点实验室,合肥,230036 |
| 基金项目： | 安徽省高校省级自然科学项目，安庆市重点科技项目，安庆师范学院科研启动项目 |
| 摘 要： | 通过氯化钙法制备大肠杆菌DH5α菌株感受态,讨论了不同保存温度和保存时间对感受态转化率的影响。结果表明,在4℃下保存,8h达到最高转化率;在-20℃和-70℃下保存,均为48h达到最高转化率。通过氯化钙法制备的DH5α菌株感受态细胞,在-20℃条件下简单保存,20d内完全可以满足一般转化研究的要求,不需要复杂的甘油、液氮处理及超低温要求。 |
| 关 键 词： | 大肠杆菌 DH5α菌株 感受态 转化率 |
Study on variation of transformation rate of E.coli DH5α competent cells |
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| XIE Ling,CHENG Hong-mei,YIN Chong,XIANG Xiao-yan,HUANG Bo.Study on variation of transformation rate of E.coli DH5α competent cells[J].Journal of Biology,2011,28(4):4-6. | |
| Authors: | XIE Ling CHENG Hong-mei YIN Chong XIANG Xiao-yan HUANG Bo |
| Institution: | XIE Ling1,2,CHENG Hong-mei1,YIN Chong 1,XIANG Xiao-yan1,HUANG Bo2(1.School of Life Sciences,Anqing Teachers College,Anqing 246011,2.Anhui Provincial Key Laboratory of Microbial Pest Control,Anhui Agricultural University,Hefei 230036,China) |
| Abstract: | The competent cells of E.coli DH5α strain were prepared by calcium chloride,and the variation of transformation rate for different treatment temperatures and times was detected.Experimental results showed that the transformation rate of competent cells stored at 4℃ reached maximum for 8 hours.And the transformation rates reached maximum for 48 hours,when they were stored at-20℃ and-70℃.The competent cells of E.coli DH5α strain prepared by calcium chloride could satisfy general research needs when they were stored directly at-20℃ within 20 days. |
| Keywords: | Escherichia coli DH5α strain competence transformation rate |
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