Synthesis,Biophysical, and Biochemical Properties of PNA-DNA Chimeras

Abstract

Three PNA-DNA chimeric dimer synthons (tT, upT and uhT, see Sch. 1) have been synthesized in solution and used to make T₂₀-analogue chimeras applying standard solid-phase DNA synthesis protocol. Duplex forming ability of chimeras with dA₂₀ and their hydrolyses by 3′- and 5′-exonucleases (snake venom and bovine spleen phosphodiesterase, respectively) have been investigated.