Sister chromatid exchanges in barley |
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| Authors: | I Schubert G Künzel H Bretschneider R Rieger H Nicoloff |
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| Institution: | (1) Zentralinstitut für Genetik und Kulturpflanzenforschung der Akademie der Wissenschaften der DDR, Gatersleben, DDR;(2) Institute of Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria |
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| Abstract: | Summary A mean frequency of 20.6 sister chromatid exchanges (SCEs) per cell has been observed in a reconstructed karyotype of Hordeum vulgare by application of the FPG technique after unifilar incorporation of BrdU into chromosomes. The involvement in SCEs of the 48 segments into which the chromosome set had been subdivided was, with a single deviation, length proportional and independent of the segment's heterochromatin content. Asymmetric bands, indicative of an uneven distribution of adenine and thymidine between the DNA strands in adenine (A)-thymidine (T) rich chromosome regions, could not be detected after incubation of the cells in BrdU for one cycle of DNA replication. |
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| Keywords: | SCE Intrachromosomal distribution of SCEs Barley |
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