Genetically altering the expression of neutral trehalase gene affects conidiospore thermotolerance of the entomopathogenic fungus Metarhizium acridum |
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Authors: | Yajun Leng Guoxiong Peng Yueqing Cao and Yuxian Xia |
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Institution: | (1) Genetic Engineering Research Center, College of Bioengineering, Chongqing University, Chongqing, 400030, China;(2) Chongqing Engineering Research Center for Fungal Insecticides, Chongqing, 400030, China;(3) Key Lab of Functional Gene and Regulation Technologies under Chongqing Municipal Education Commission, Chongqing, 400030, China |
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Abstract: | Background The entomopathogenic fungus Metarhizium acridum has been used as an important biocontrol agent instead of insecticides for controlling crop pests throughout the world. However,
its virulence varies with environmental factors, especially temperature. Neutral trehalase (Ntl) hydrolyzes trehalose, which plays a role in environmental stress response in many organisms, including M. acridum. Demonstration of a relationship between Ntl and thermotolerance or virulence may offer a new strategy for enhancing conidiospore thermotolerance of entomopathogenic
fungi through genetic engineering. |
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