Analysis of the DNA Marker Specific to the Wheat G Genome |
| |
| Authors: | A B Shcherban E K Khlestkina E A Salina |
| |
| Institution: | (1) Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090, Russia |
| |
| Abstract: | A RAPD marker specific for the G genome of wheat was identified. The corresponding 1171-bp DNA sequence was cloned and analyzed. Screening of the database did not reveal any homologies with the known plant DNA sequences. Using the primers specific to the flanking regions of the marker sequence, PCR analysis of the polyploid wheat species and the diploid species of the section Sitopsis was carried out. In addition, using the cloned sequence as a molecular hybridization probe, RFLP analysis of the genomic DNA of these species was performed. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
