Development of a composite map in Vicia faba,breeding applications and future prospects |
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| Authors: | Román B Satovic Z Pozarkova D Macas J Dolezel J Cubero J I Torres A M |
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| Institution: | (1) Departamento de Mejora y Agronomia CIFA-Alameda del Obispo, Apdo 3092, 14080 Córdoba, Spain;(2) Faculty of Agriculture. Department of Seed Science and Technology, Svetosimunska 25, 10000 Zagreb, Croatia;(3) Institute of Plant Molecular Biology, Branisovska 31, 37005 Ceske Budejovice, Czech Republic;(4) Institute of Experimental Botany, Sokolovská 6, 77200 Olomouc, Czech Republic;(5) Departamento de Genética, E.T.S.I.A.M., Apdo 3048, 14080 Córdoba, Spain |
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| Abstract: | A composite map of the Vicia faba genome based on morphological markers, isozymes, RAPDs, seed protein genes and microsatellites was constructed. The map incorporates data from 11 F2 families for a total of 654 individuals all sharing the common female parent Vf 6. The integrated map is arranged in 14 major linkage groups (five of which were located in specific chromosomes). These linkage groups include 192 loci and cover 1,559 cM with an overall average marker interval of 8 cM. By joining data of a new F2 population segregating for resistance to ascochyta, broomrape and others traits of agronomic interest, have been saturated new areas of the genome. The combination of trisomic segregation, linkage analysis among loci from different families with a recurrent parent, and the analysis of new physically located markers, has allowed the establishment of the present status of the V. faba map with a wide coverage. This map provides an efficient tool in breeding applications such as disease-resistance mapping, QTL analyses and marker-assisted selection.Communicated by J.W. Snape |
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