首页 | 本学科首页   官方微博 | 高级检索  
     


Shoot Organogenesis from Immature Zygotic Embryo Cultures of Ginkgo biloba
Authors:P.-S. Choi  D.-Y. Cho  W.-Y. Soh
Affiliation:(1) Eugentech Inc./Bioventure, Korea Research Institute of Bioscience and Biotechnology, Tajeon, 305-333, Korea;(2) Department of Biological Sciences, Chonbuk National University, Chonju, 561-756, Korea;(3) Department of Biology, Chonju Woosuk University, Chonju, 565-701, Korea
Abstract:Immature zygotic embryos were cultured on Murashige and Skoog's medium (MS) supplemented with various combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid (NAA), benzyladenine (BA) and zeatin or with various concentrations of 2,4-D alone. The maximum number (8 per embryo) of adventitious buds formed from cotyledons of heart stage embryos cultured on MS medium with 1 mg dm−3 BA and 0.01 mg dm−3 NAA. The adventitious buds originated from procambial strands of immature embryo cotyledons and then developed into adventitious bud primordia within 20 d. Adventitious buds transferred to hormone free MS medium grew into shoots, but did not produce plantlets because the shoots failed to root. This revised version was published online in July 2006 with corrections to the Cover Date.
Keywords:adventitious shoot formation  benzyladenine  2,4-dichlorophenoxyacetic acid  naphthaleneacetic acid  zeatin
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号