| EB病毒融合基因重组BCG穿梭质粒的构建及表达 |
| |
| 引用本文： | 薛庆节,陈廷,朱伟.EB病毒融合基因重组BCG穿梭质粒的构建及表达[J].微生物学免疫学进展,2010,38(2):24-27. |
| |
| 作者姓名： | 薛庆节 陈廷 朱伟 |
| |
| 作者单位： | 济宁医学院日照校区微生物学教研室,日照,276826 |
| |
| 基金项目： | 山东省卫生厅基金，济宁医学院青年基金 |
| |
| 摘 要： | 分别以卡介苗(BCG)和EB病毒融合基因cDNA为模板,通过PCR扩增得到139bp的BCG-Ag85B信号肽序列和2291bp的Z2A基因序列。将BCG-Ag85B信号肽序列与大肠杆菌-卡介苗穿梭表达载体pMV261重组,得到重组质粒pMVS。再将EB病毒融合基因序列Z2A亚克隆至pMVS中,得到重组质粒pMVZ2A,电转化导入BCG。SDS-PAGE分析结果表明,构建的重组质粒pMVZ2A经双酶切、PCR扩增及测序鉴定证实,克隆基因BCG-Ag85B信号肽和Z2A正确插入载体pMV261,电转化导入BCG,能够在BCG中分泌表达。
|
| 关 键 词： | 卡介苗 融合基因Z2A 基因重组 |
Construction and its expression of recombinant BCG shuttle-plasmid of EB virus fused gene |
| |
| XUE Qing-jie,CHEN Ting,ZHU Wei.Construction and its expression of recombinant BCG shuttle-plasmid of EB virus fused gene[J].Progress In Microbiology and Immunology,2010,38(2):24-27. |
| |
| Authors: | XUE Qing-jie CHEN Ting ZHU Wei |
| |
| Institution: | (Department of microbiology, fining Medical college ,Rizhao , 276826, China ) |
| |
| Abstract: | In this research ,BCG Ag85B signal sequence and Z2A gene were amplified from the genome of BCG and Z2A by PCR, respectively. BCG Ag85B signal sequence was cloned into E. coli-BCG shuttle-vector pMV261 to obtain pMVS, then a new recombinant plasmid pMVZ2A was constructed by inserting the Z2A gene into pMVS and transformed into BCG by electrntransformation. The expressed products were analyzed by SDS-PAGE. The tested results showed that the cloned genes BCG Ag85B and Z2A are correctly inserted into the vector pMV261 ,which have been confirmed by restriction endonuclease digestion and PCR amplification of Z2A and gene sequencing, respectively. They have been transformed into BCG and secretory expressed. |
| |
| Keywords: | BCG Z2A recombinant Gene recombination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
