Immortalized mesenchymal stem cells: an alternative to primary mesenchymal stem cells in neuronal differentiation and neuroregeneration associated studies |
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| Authors: | Min Gong Yang Bi Wei Jiang Yun Zhang Li Chen Nali Hou Youxue Liu Xiaoping Wei Jie Chen Tingyu Li |
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| Affiliation: | (1) Children’s Nutrition Research Center, Children’s Hospital of Chongqing Medical University, Chongqing, 400014, China;(2) Ministry of Education Key Laboratory of Child Development and Disorders; Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, 400014, China |
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| Abstract: | Background Mesenchymal stem cells (MSCs) can be induced to differentiate into neuronal cells under appropriate cellular conditions and transplanted in brain injury and neurodegenerative diseases animal models for neuroregeneration studies. In contrast to the embryonic stem cells (ESCs), MSCs are easily subject to aging and senescence because of their finite ability of self-renewal. MSCs senescence seriously affected theirs application prospects as a promising tool for cell-based regenerative medicine and tissue engineering. In the present study, we established a reversible immortalized mesenchymal stem cells (IMSCs) line by using SSR#69 retrovirus expressing simian virus 40 large T (SV40T) antigen as an alternative to primary MSCs. |
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| Keywords: | mesenchymal stem cells reversible immortalization simian virus 40 large T cell senescence neuronal differentiation cell transplantation hypoxic-ischemic brain damage |
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