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A Recombinant Protein Based on Trypanosoma cruzi P21 Enhances Phagocytosis |
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| Authors: | Adele A Rodrigues Tatiana M Clemente Marlus A dos Santos Fabrício C Machado Rafael G B Gomes Heline Hellen T Moreira Mário C Cruz Paula C Brígido Paulo C F dos Santos Flávia A Martins Diana Bahia Juliana T Maricato Luiz M R Janini Eduardo H Reboredo Renato A Mortara Claudio V da Silva |
| Institution: | 1. Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Brazil.; 2. Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina - Universidade Federal de São Paulo, São Paulo, Brazil.; 3. Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, Brazil.; Louisiana State University, United States of America, |
| Abstract: | BackgroundP21 is a secreted protein expressed in all developmental stages of Trypanosoma cruzi. The aim of this study was to determine the effect of the recombinant protein based on P21 (P21-His6) on inflammatory macrophages during phagocytosis.FindingsOur results showed that P21-His6 acts as a phagocytosis inducer by binding to CXCR4 chemokine receptor and activating actin polymerization in a way dependent onthe PI3-kinase signaling pathway.ConclusionsThus, our results shed light on the notion that native P21 is a component related to T. cruzi evasion from the immune response and that CXCR4 may be involved in phagocytosis. P21-His6 represents an important experimental control tool to study phagocytosis signaling pathways of different intracellular parasites and particles. |
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