Plantlet regeneration from protoplast-derived haploid embryogenic calli of wheat |
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Authors: | Gu Xu Liang George H |
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Institution: | (1) Genetics Program, Department of Agronomy, Kansas State University, Manhattan, KS 66506-5501, USA |
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Abstract: | To search for an alternative method for protoplast culture, regenerable embryogenic calli were obtained from anther culture
of three wheat cultivars, Karl 92, Jinghua #1, and Pavon 76. Protoplasts were isolated directly from the haploid embryogenic
calli and cultured in modified PMI and LM8P media without going through cell suspension culture. After 8–11 days of subculture,
the embryogenic calli produced the maximum yield of protoplasts and cell division was at the highest frequency when plated
at a density of 3–4 × 105 protoplasts ml−1. Frequency of colony formation varied from 0.2% to 0.5% for Jinghua #1 and from 0.1% to 2% for Pavon 76, while Karl 92 failed
to produce colonies, even though its embryogenic calli were friable and fast-growing on the maintenance medium. Green haploid
plantlets of Jinghua #1 and Pavon 76 have been regenerated from protoplasts, which were cultured on a differentiation medium
first and then on a rooting medium.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | anther culture cell density protoplast Triticum aestivum L |
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