| JDV Tat反式激活LTR与HIV-1 Tat采用类似的细胞因子 |
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| 引用本文： | 邓刚,莎日娜,母俊杰,乔文涛,耿运琪,陈启民. JDV Tat反式激活LTR与HIV-1 Tat采用类似的细胞因子[J]. Virologica Sinica, 2004, 19(6): 576-581 |
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| 作者姓名： | 邓刚 莎日娜 母俊杰 乔文涛 耿运琪 陈启民 |
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| 作者单位： | 南开大学生命科学学院,天津300071 |
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| 基金项目： | 国家自然科学基金(30270059) |
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| 摘 要： | 为分析JDV Tat在反式激活JDV及HIV-1 LTR过程中是否采用与H1V-1 Tat类似的细胞因子，本文构建了包含完整激活域的jTat70和hTat47，同时构建了cyclin T1和CDK9真核表达及反义转译质粒。过量表达hTat47和jTat70对hTat反式激活HIV-1 LTR，jTat反式激活JDV和HIV-1 LTR均有明显的抑制作用推测jTat和hTat的反式激活作用可能涉及类似的细胞因子。通过cyclin T1和CDK9的反义转译质粒对jTat反式激活的抑制作用证实这两种细胞因子参与了jTat对JDV和HIV-1 LTR的反式激活。
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| 关 键 词： | Jembrana病毒 人免疫缺陷病毒 反式激活因子 细胞周期蛋白T1 细胞周期蛋白依赖性的激酶9 |
JDV Tat Transactivate LTR Involves Similar Cytokines as HIV-1 Tat |
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| DENG Gang,SHA Ri-na,MU Jun-jie,Qiao Wen-tao,GENG Yun-qi,CHEN Qi-min. JDV Tat Transactivate LTR Involves Similar Cytokines as HIV-1 Tat[J]. 中国病毒学(英文版), 2004, 19(6): 576-581 |
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| Authors: | DENG Gang SHA Ri-na MU Jun-jie Qiao Wen-tao GENG Yun-qi CHEN Qi-min |
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| Affiliation: | DENG Gang,SHA Ri-na,MU Jun-jie,Qiao Wen-tao,GENG Yun-qi,CHEN Qi-min** |
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| Abstract: | In order to define if similar cytokines take part in transactivation of Jembrana disease virus and Human immunodeficiency virus-1 Tat protein, two truncated Tat proteins, hTat47 and jTat70, which contained intact activation domains but lacking binding domains, were constructed. We also constructed cyclin T1 and CDK9 eukaryotic expression and antisense plasmids. Overexpression of hTat47 and jTat- 70 both inhibited transactivation of JDV and HIV-1 Tat on HIV-1 or JDV LTR, suggesting that they may involve similar cytokines. Antisense transient assay of cyclin T1 and CDK9 further proved that these cytokines also participated in transactivation of JDV Tat. |
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| Keywords: | JDV HIV-1 Tat Cyclin T1 CDK9 |
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