| 家蚕微粒子病原虫(Nosema bombycis)小亚基核糖体RNA全基因的克隆及其二级结构的构建 |
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| 引用本文： | 王见杨,黄可威,陆长德.家蚕微粒子病原虫(Nosema bombycis)小亚基核糖体RNA全基因的克隆及其二级结构的构建[J].昆虫学报,2002,45(3):290-295. |
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| 作者姓名： | 王见杨 黄可威 陆长德 |
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| 作者单位： | 1. 中国农业科学院蚕业研究所,镇江,212018
2. 中国科学院上海生物化学研究所,上海,200031 |
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| 基金项目： | 国家“九五”科技攻关专题 ( 96 6 16 0 2 0 3)，江苏省“九五”科技攻关课题 (BE96 36 9 1) |
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| 摘 要： | 在用PCR技术扩增、克隆、测序了家蚕微粒子病原虫Nosema bombycis (镇江株)小亚基核糖体RNA基因核心序列(5'-端起1 200 bp)的基础上，用SSP-PCR技术克隆了核心序列3'-端下游序列，从而获得了家蚕微粒子病原虫小亚基核糖体RNA基因的全序列共1 233 bp。 用RnaViz 、Forcon、DCSE等生物软件构建了家蚕微粒子病原虫小亚基核糖体RNA的二级结构，与其它微孢子虫及真核生物小亚基核糖体RNA的二级结构相比，该二级结构缺乏螺旋10、E10-1、11、18、E23-n和43。
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| 关 键 词： | 家蚕微粒子病原虫 SSP-PCR 小亚基核糖体RNA(SSUrRNA)全基因 二级结构 螺旋 |
| 文章编号： | 0454-6296(2002)03-0290-06 |
| 修稿时间： | 2000年10月16 |
Analyses of small subunit ribosomal RNA sequence of the microsporidium, Nosema bombycis and its secondary structure |
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| WANG Jian-Yang ,HUANG Ke-Wei ,LU Chang-De.Analyses of small subunit ribosomal RNA sequence of the microsporidium, Nosema bombycis and its secondary structure[J].Acta Entomologica Sinica,2002,45(3):290-295. |
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| Authors: | WANG Jian-Yang HUANG Ke-Wei LU Chang-De |
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| Institution: | WANG Jian-Yang 1,HUANG Ke-Wei 1,LU Chang-De 2 |
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| Abstract: | The nucleotide sequence (1 205 bp) of small subunit ribosomal RNA (SSUrRNA) of the microsporidium, Nosema bombycis (Zhenjiang), was amplified by polymerase chain reaction(PCR). Its 3'-end was obtained using the single specific primer-PCR technique with the primers matching the highly conserved parts of SSUrRNA genes cloned. The entire coding region for SSUrRNA of N. bombycis (Zhenjiang) was 1 233 bp as described elsewhere. The sequence shared high homology with those of many microsporidia, especially N. apis. The secondary structure of the SSUrRNA was constructed with RnaViz, Forcon and DCSE. There were no helices such as 10, E10-1, 11,18, E23-n and 43 in it. |
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| Keywords: | Nosema bombycis SSP-PCR SSUrRNA gene secondary structure helix |
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