| 尖孢镰孢一新硝酸盐营养突变类型与营养体亲和性 |
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| 引用本文： | 鲍建荣,郑重,刘惠君.尖孢镰孢一新硝酸盐营养突变类型与营养体亲和性[J].菌物学报,1993,12(4). |
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| 作者姓名： | 鲍建荣 郑重 刘惠君 |
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| 作者单位： | 浙江农业大学梧保系，杭州 310029 |
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| 摘 要： | 从73个尖孢镰孢(Fusarium oxysporum)不同专化型菌株上获得684个硝酸盐营养突变株(nit mutant)。作相关氮源利用试验及亚硝酸反应后,鉴定出一新硝酸盐营养突变类型:亚硝酸盐还原酶结构基因类型,命名为nit8,占总突变株的6.7%。同时被鉴别的还有nit1、nit3和Nit M三种突变类型,它们分别占突变株总数的81.0%,3.8%和8.5%。此外,首次引入一种亚硝酸反应在这类研究中的应用,还提出了互补指数概念与公式来表示nit突变株营养体之间亲和的能力。
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| 关 键 词： | 尖孢镰孢 硝酸盐营养突变株 突变类型 互补指数 |
VEGETATIVE COMPATIBILITY AND A NEW NITRATE NONUTILIZING MUTANT PHENOTYPE IN FUSARIUM OXYSPORUM |
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| Authors: | Bao Jian-rong Zheng Zhong Lui Hui-jun |
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| Institution: | Bao Jian-rong,Zheng Zhong,Lui Hui-junDepartment of Plant Protection,Zhejiang Agricultural University,Hangzhou 310029 |
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| Abstract: | A new nitrate nonutilizing (nit)mutant phenotype,named nit 8, reflecting mutation presumptively in a structural gene for nitrate reductase was de- termined in Fusarium oxysporum isolates.In addition to nit 8,phenotypes nit 1, nit 3 and Nit M were also identified among 684 nit mutants discovered from 73 isolates of F.oxysporum and their isolation frequencies were 6.7,81.0,3.8 and 8.5%, respectively.A color indicating method in nitrite excretion tests was firstly intro- duced in such kind of studies and a new concept with a formula on complementation index (CI) was proposed to express the vegetative compatibility in pairing tests be- tween nit mutants. |
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| Keywords: | Fusarium oxysporum Nitrate nonutilizing mutant Phenotype Vegetative compatibility Complementation index |
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