|
|||||
|
|
Characterization of cellulolytic enzymes and bioH2 production from anaerobic thermophilic Clostridium sp. TCW1 |
|
| Authors: | Lo Yung-Chung Huang Chi-Yu Cheng Chieh-Lun Lin Chiu-Yue Chang Jo-Shu |
| Institution: | a Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan b Department of Environmental Engineering and Science, Tunghai University, Taichung 407, Taiwan c Department of Environmental Engineering and Science, Feng Chia University, Taichung, Taiwan d Sustainable Environment Research Center, National Cheng Kung University, Tainan, Taiwan e Center for Biosciences and Biotechnology, National Cheng Kung University, Tainan, Taiwan f Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan, Taiwan |
| Abstract: | A thermophilic anaerobic bacterium Clostridium sp. TCW1 was isolated from dairy cow dung and was used to produce hydrogen from cellulosic feedstock. Extracellular cellulolytic enzymes produced from TCW1 strain were identified as endoglucanases (45, 53 and 70 kDa), exoglucanase (70 kDa), xylanases (53 and 60 kDa), and β-glucosidase (45 kDa). The endoglucanase and xylanase were more abundant. The optimal conditions for H2 production and enzyme production of the TCW1 strain were the same (60 °C, initial pH 7, agitation rate of 200 rpm). Ten cellulosic feedstock, including pure or natural cellulosic materials, were used as feedstock for hydrogen production by Clostridium strain TCW1 under optimal culture conditions. Using filter paper at 5.0 g/L resulted in the most effective hydrogen production performance, achieving a H2 production rate and yield of 57.7 ml/h/L and 2.03 mol H2/mol hexose, respectively. Production of cellulolytic enzyme activities was positively correlated with the efficiency of dark-H2 fermentation. |
| Keywords: | Biohydrogen Cellulosic feedstock Clostridium sp Enzymatic hydrolysis Dark fermentation |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |