Endcaps for stabilizing short DNA duplexes |
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| Authors: | Ng Pei-Sze Pingle Maneesh R Balasundarum Ganesan Friedman Alan Zu Xiaolin Bergstrom Donald E |
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| Institution: | Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907-1333, USA. |
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| Abstract: | The syntheses of endcaps for covalently linking the 3' and 5' hydroxyl groups of blunt end double-stranded DNA are described. Endcap diols were converted into DMTr protected phosphoramidites and incorporated between nucleotides 4 and 5 of a self-complementary octamer. The stabilizing effect of the endcaps on duplex DNA was determined by Tm experiments on the self-complementary octamer. |
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