Interactions of trimeric purine nucleoside phosphorylases with ground state analogues--calorimetric and fluorimetric studies |
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Authors: | Wielgus-Kutrowska Beata Frank Joachim Holý Antonin Koellner Gertraud Bzowska Agnieszka |
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Institution: | Department of Biophysics, Institute of Experimental Physics, University of Warsaw, Warsaw, Poland. |
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Abstract: | Binding enthalpies, dissociation constants and stoichiometry of binding for interaction of trimeric calf spleen and Cellulomonas sp. purine nucleoside phosphorylases with their ground state analogues (substrates and inhibitors) were studied by calorimetric and spectrofluorimetric methods. Data for all ligands, with possible exception of hypoxanthine, are consistent with three identical non-interacting binding sites. |
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