Purification of human leucocyte DNA: proteinase K is not necessary. |
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| Authors: | A M Douglas A M Georgalis L R Benton K L Canavan B A Atchison |
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| Affiliation: | Victorian Institute of Forensic Pathology, South Melbourne, Australia. |
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| Abstract: | ![]()
A rapid nontoxic method for the purification of DNA from human leucocytes is described. Preliminary experiments which tested different methods of DNA purification indicated that digestion of proteins with proteinase K was unnecessary. This led to the development of a simple procedure involving lysis of the cells in SDS followed by extraction with 6 M NaCl. The method described overcomes the requirement for lengthy incubations in the presence of expensive proteinase K and subsequent extraction with toxic chemicals. |
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