Purification, crystallization, and X-ray diffraction studies of lactotransferrin from buffalo colostrum. |
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Authors: | A Raman K L Bhatia T P Singh A Srinivasan C Betzel R C Malik |
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Institution: | Department of Biophysics, All India Institute of Medical Sciences, New Delhi. |
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Abstract: | Lactotransferrin is an iron-binding protein. It has been purified from buffalo colostrum. The purified lactotransferrin has been crystallized in 10% ethanol solution. The crystals are orthorhombic and the space group is P2(1)2(1)2(1) with unit cell dimensions a = 161.70 A, b = 155.75 A, c = 113.48 A. The asymmetric unit contains three molecules of the protein with a solvent content of about 59%. The crystals were stable in the X-ray beam and diffract beyond 3.5 A resolution. The native data have been collected and the structure determination is in progress. |
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