| JC病毒衣壳蛋白VP1多克隆抗体的制备 |
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| 引用本文： | 黄敏,杨琼,李炜,王琦,成军.JC病毒衣壳蛋白VP1多克隆抗体的制备[J].中国微生态学杂志,2010,22(10):884-885,893. |
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| 作者姓名： | 黄敏 杨琼 李炜 王琦 成军 |
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| 作者单位： | [1]大连医科大学微生物教研室,辽宁大连116044 [2]北京地坛医院传染病研究所,北京100015 |
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| 摘 要： | 目的制备pET-32a(+)-VP1蛋白的多克隆抗体。方法用纯化后的VP1蛋白分4次免疫兔子,颈动脉插管法取血,制得多克隆抗体。结果用ELISA法和Western blot鉴定多克隆抗体的效价得1?320000。该抗体可以用Western blot法检测出59 kD左右的VP1蛋白。结论成功制备高效价的JC病毒衣壳蛋白VP1多克隆抗体。
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| 关 键 词： | JC病毒 VP1蛋白 多克隆抗体 |
Preparation of JC virus capsid protein-VP1 polyclonal antibody |
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| HUANG Min,YANG Qiong,LI Wei,WANG Qi,CHENG Jun.Preparation of JC virus capsid protein-VP1 polyclonal antibody[J].Chinese Journal of Microecology,2010,22(10):884-885,893. |
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| Authors: | HUANG Min YANG Qiong LI Wei WANG Qi CHENG Jun |
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| Institution: | 1.Department of Microbiology,Dalian Medical University,Dalian 116044,China;2.Beijing Ditan Hospital,Beijing 100015,China) |
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| Abstract: | Objective To preparate the pET-32a（＋）-VP1 polyclonal antibody.Method Rabbits were immuned with purified VP1 four times.Polyclonal antibody was made by carotid artery intubation and withdrawing blood.Result The titer of polyclonal antibody was 1︰320000 detected by ELISA and Western blot.VP1 protein with the molecular weight at approximate 59 kD had been detected by Western blot.Conclusion The study successfully prepare JC virus capsid protein-VP1 polyclonal antibody with high titer. |
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| Keywords: | JC virus VP1 protein Polyclonal antibody |
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